The Institutional Animal Care and Use Committee and DCM have updated the mouse breeding and cage density policy at SUNY Downstate. This updated policy includes a review of the benefits of the two most commonly used breeding schemes while also ensuring that our biomedical research community complies with the national standards and recommendations contained within the 8th Edition of the Guide for the Care and Use of Laboratory Animal (NRC 2011).
Monogamous or Pair Breeding = ONE (1) adult male and ONE (1) adult female.
Trio Breeding = ONE (1) adult male and TWO (2) adult females.
* note only one male is permitted per cage.
The USDA Animal Welfare Act Regulations (AWARs) and the Public Health Service Policy on the Humane Care and Use of Laboratory Animals (PHS Policy) stipulate that the IACUC must conduct continuing reviews of previously approved research activities. 
Conducting research activities  under a protocol that has expired constitutes a serious violation of both the AWARs and PHS Policy, and is therefore unacceptable under any circumstance. Upon expiration of an approved animal activity, the Principal Investigator (PI) will be notified that all animal-related research activities covered by that protocol must be stopped in accordance with PHS and IACUC Policy. In order to prevent lapses in protocol coverage, animals attached to the expired protocol, whether naïve or part of an active experiment, shall be transferred to the DCM Holding Protocol and care provided per DCM routine SOPs or as described in the holding protocol. PI and research study staff access to these animals will be restricted and limited to DCM staff only during the period of protocol expiration. Animals assigned to the Holding Protocol may be returned to the PI's control only after submission and approval of an IACUC protocol.
PHS Funded Studies: The Office of Management and Budget Cost Principles and the NIH Grants Policy Statement (NIHGPS) do not permit use of contract or grant funds for the conduct of animal activities during a lapse in IACUC approval or suspension of animal activities . If animal activities are expected to resume at a later time, please contact the DCM Office Manager at ext. 3812 to provide an account number that is not tied to federal funds. Costs incurred during the period of expiration or suspension related to maintenance and care of animals will be billed to the non-PHS account at a rate 2 times higher than the usual per diem rate.
 USDA AWARs § 2.31(d)(5) and PHS Policy IV.C.5
 "Research activities" includes any experimental manipulations (e.g., surgery, collection of blood, collection of tissue for genotyping, behavioral testing), special housing conditions (e.g., special diet or feeding/watering schedule, special light cycle, special cage changing cycle), collection of tissues at euthanasia for postmortem analysis, setting up breeding pairs, or any other collection of data from animals under that protocol.
 NIH Guidance Document NOT-OD-07-044